《植物生理学报》 2009, 45(5): 449-454

灰绿藜液泡膜焦磷酸酶基因(CgVP1)过表达提高拟南芥的耐盐性

胡有贞, 王瑜, 张富春*

新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐830046

通信作者：张富春；E-mail: zfcxju@xju.edu.cn；Tel: 0991-8583517

摘　要：

克隆获得包含完整开放阅读框(O R F)的灰绿藜液泡膜焦磷酸酶基因(CgV P1 ) cD N A 序列, 构建成基因表达载体 pCAMBIA1301.1-CgVP1 后, 利用根癌农杆菌介导的花序浸染法转化拟南芥, 再以抗性筛选方法获得了T3 代纯合的转基因 植株, 经检测外源目的基因已经整合到拟南芥基因组中并能正常表达。分析结果表明, 在拟南芥中过表达CgVP1基因后提 高了植株抗盐胁迫的能力。

关键词：灰绿藜; 液泡膜焦磷酸酶基因; 过表达; 耐盐性

收稿：2009-01-09   修定：2009-02-03

资助：新疆维吾尔自治区科技重大专项(200731138-3)和教育部科学技术研究重点项目(205178)。

Overexpression of Chenopodium glaucum Tonoplast Pyrophosphatase (CgVP1) Improves Salt Tolerance in Arabidopsis thaliana

HU You-Zhen, WANG Yu, ZHANG Fu-Chun*

Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang

Corresponding author: ZHANG Fu-Chun; E-mail: zfcxju@xju.edu.cn; Tel: 0991-8583517

Abstract:

The open reading frame (ORF) of halophyte Chenopodium glaucum tonoplast pyrophosphatase gene (CgVP1) was successfully cloned and inserted into plant expression vector pCAMBIA1301.1, then the recombinant (pCAMBIA1301.1-CgVP1) was transformed into Arabidopsis thaliana through floral dipping method, which was mediated by Agrobacterium tumefaciens. Homozygous T₃ transgenic plants that were resistant to hygromycin were obtained. The results indicated that CgVP1 gene was integrated into Arabidopsis thaliana genome and expressed normally. Various salt tolerance analysis showed that that overexpression of tonoplast pyrophosphatase gene from Chenopodium glaucum could improve the salt tolerance in transgenic Arabidopsis thaliana.

Key words: Chenopodium glaucum; tonoplast pyrophosphatase; overexpression; salt tolerance